You have to connect the serial bus cable from the uC to the Raspberry. It was connected to the lower middle USB-Port in this case, 1. start bash (2. workon cv-4.5.3.56) 3. python 4. import serial 5. s = serial.Serial('/dev/ttyACM0', 9600) Now you can control the LEDs. To let the stripe light up in one color: s.write(str.encode('RRR-GGG-BBB')) eg: s.write(str.encode('255-000-000')) To let the stripe light up in one of the 20 color patterns: s.write(str.encode('color_pattern_XX')), where x is 01-20 eg: s.write(str.encode('color_pattern_01')) Now you can start a program to detect the leds